The selection of representative cell lines for expressing bispecific antibodies (BsAbs) is a crucial initial stage that significantly influences overall process yield and quality. The chosen cell lines represent the basic productivity and protein quality of the BsAb, impacting the potential for subsequent process development, scalability, stability, and purity of the main product. Because the structure of a BsAb is more complex than that of antibodies, it will also encounter more production problems in manufacturing, such as low product yield, erroneous chain assembly, and high aggregate levels. Therefore, the extensive screening and evaluation of multiple BsAb-expressing cell clones at this stage are necessary and play a pivotal role. Having a suitable clone can reduce the problems that upstream and downstream processes may face. Optimizing the expression plasmid and improving the screening strategies can help us obtain the best production clone. 

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